Learn More
BD Pharmingen™ MitoStatus Red
Description
In cells undergoing apoptosis, pro-apoptotic Bcl-2 family proteins cause mitochondrial outer membrane permeabilization (MOMP), resulting in the release of cytochrome C and the subsequent activation of caspase-9 and the apoptotic cascade. MOMP often correlates with the loss of inner mitochondrial membrane potential (ΔΨm), which can be detected using ΔΨm-sensitive dyes. These cationic, lipophilic dyes accumulate within the mitochondria of healthy cells, but not within mitochondria that have lost ΔΨm due to induction of apoptosis or treatment with a mitochondrial uncoupler.
BD Pharmingen™ MitoStatus Red is a fluorescent dye that is readily sequestered by active mitochondria, allowing for flow cytometric or imaging analysis to assess for apoptosis or mitochondrial depolarization. Apoptotic or cells with depolarizard mitochondria will have diminished levels of fluorescence. MitoStatus Red has an excitation maximum of 622nm and an emission maximum of 648nm.
Spécification
Spécification
| Quantity | 100 μg |
| Test Specificity | Tested in Development: Human, Mouse |
| Content And Storage | Upon arrival, store the dye powder desiccated and protected from light at ≤ -20°C until use. The dye powder is stable for at least 12 months if stored as indicated. After reconstitution with DMSO, store the stock solution at ≤ -20°C in small aliquots. The stock solution is stable for at least 6 months if stored as indicated. |
En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.