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  6. Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

Product Code. 15889856 Shop All Applied Biosystems Products
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No. of Reactions:
100 Reactions
1000 Reactions
24 Reactions
Unit Size:
100 reactions
1000 reactions
24 reactions
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Product Code. No. of Reactions unitSize
15889856 100 Reactions 100 reactions
16335342 1000 Reactions 1000 reactions
15899856 1000 Reactions 1000 reactions
16325342 100 Reactions 100 reactions
16345342 24 Reactions 24 reactions
15809866 24 Reactions 24 reactions
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Product Code. 15889856 Supplier Applied Biosystems™ Supplier No. 4458687

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Description

Includes

This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

  • A streamlined, simple procedure
  • Performance of all sequencing steps in a single reaction tube
  • Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
  • Substantial time savings versus previous workflows

Order Info

1 Kit

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage This kit contains BigDye™ Direct PCR Master Mix, BigDye™ Direct Sequencing Master Mix, BigDye™ Direct M13 Fwd Primer, BigDye™ Direct M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 100 reactions.
Format 8-well Strip, 96-well Plate
For Use With (Equipment) 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer
For Use With (Application) Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Includes BigDye™ Direct Cycle Sequencing Kit, 100 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler
No. of Reactions 100 Reactions
Product Line BigDye
Product Type Direct Cycle Sequencing Kit
Quantity 100 reactions
Shipping Condition Dry Ice
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
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For Research Use Only. Not for use in diagnostic procedures.

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