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Calnexin Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Brand: Invitrogen PA534754
Additional Details : Weight : 0.01000kg
Description
Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, KN73. Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Pig (100%), Bovine (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).Specifications
Calnexin | |
Polyclonal | |
Unconjugated | |
CANX | |
1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
101122273, 12330, 29144, 821 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
0.46 mg/mL | |
PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7 | |
P27824, P35564, P35565 | |
CANX | |
Synthetic peptide corresponding to a region within amino acids 525 and 592 of Calnexin (Uniprot ID#P27824) | |
100 μL | |
Primary | |
Human, Mouse, Ovine, Rat | |
Antibody | |
IgG |