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Molecular Probes™ CaptAvidin™ Biotin-Binding Protein
Description
CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ∼109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Specifications
Specifications
| Conjugate | Unconjugated |
| Quantity | 1 mg |
| Content And Storage | Store in freezer (-5 to -30°C). |
| Shipping Condition | Room Temperature |
| Form | Solid |
| Product Line | CaptAvidin |
| Product Type | Avidin (unconjugated, reversible biotin binding) |
For Research Use Only. Not for use in diagnostic procedures.
Product Title
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