PeproTech CD137 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MERTRSLQDP CSNCPAGTFC DNNRNQICSP CPPNSFSSAG GQRTCDICRQ CKGVFRTRKE CSSTSNAECD CTPGFHCLGA GCSMCEQDCK QGQELTKKGC KNCCFGTFND QKRGICRPWT NCSLDGKSVL VNGTKERDVV CGPSPADLSP GASSVTPPAP AREPGHS. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human 4-1BB Receptor. Anti-Human 4-1BB Receptor-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect h4-1BB Receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human 4-1BB Receptor (500-P167G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant h4-1BB Receptor. Western Blot: To detect h4-1BB Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant h4-1BB Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P167GBT-1MG will be provided as 2 x 500 μg

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.