PeproTech CD154 (CD40 Ligand) (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human sCD40 Ligand. Anti-Human sCD40 Ligand-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human sCD40 Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD40 Ligand (500-P142G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sCD40 Ligand. Western Blot: To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsCD40L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P142GBT-1MG will be provided as 2 x 500 μg

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.