Invitrogen™ CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™

Description

The monoclonal antibody 10F7MN recognizes human glycophorin A (also known as CD235a). The antibody can see both the M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein found on the erythrocyte (RBC) and erthyroid progenitor cell membrane at about 500,000 copies per cell. The gene for glycophorin resides on chromosome 4 and has 2 allelic forms: M and N, which differ in two amino acids. The M group possesses Ser1 and Gly5 while the N group has Leu1 and Glu5. Recent data suggest that exposure to toxins can cause mutation or loss of an allele resulting in phenotypic changes. Studies are also beginning to correlate genotype/phenotype with predisposition to cancer and heart disease. Applications Reported: This 10F7MN antibody has been reported for use in flow cytometric analysis. Applications Tested: This 10F7MN antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Ultraviolet 737 is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser.

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.