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IBA Lifesciences CD3 Fab-TACS™ Agarose Column Starter Kit
Label-free isolation of CD3+ T cells
Brand: IBA Lifesciences 6-3304-002
Additional Details : CAS : 26628-22-8 Weight : 0.18600kg
Description
- Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology
- CD3 is a co-receptor that associates with the T cell receptor and plays a role in T cell activation
- Strep-Tactin™ TACS Agarose columns are filled with Strep-Tactin™ coated agarose macrobeads developed for cell isolation
- Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) bind to the matrix
- Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away
- In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps
- After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity
- The label-free authentic target cells are now ready for further downstream applications
Specifications
| Strep-Tactin™ TACS Agarose columns, 0.3 mL (2 x), CD3 Fab-Strep (mouse), 10 x Buffer CI, Biotin stock solution, TACS Column Adapter | |
| 2°C to 8°C | |
| Murine splenocytes | |
| Mouse |
For Research Use Only.