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CD3e, eFluor 450, clone: 145-2C11, eBioscience™

Armenian Hamster Monoclonal Antibody

Marque:  Affymetrix eBioscience 48-0031-80

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15361670

  • 97.00€ / 25µg


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Description

Description

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

CD3e
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P22646
FLJ18683, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide TiT3 complex, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
145-2C11
eFluor 450
Cd3e
Liquid
Cd3e
IgG
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
12501
Vidéos

For Research Use Only.