CD49b (Integrin alpha 2) Monoclonal Antibody (HMa2), Functional Grade, eBioscience™, Invitrogen™

Description

The HMalpha2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin alpha2 subunit. The complex of CD49b non-covalently associated with integrin beta1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4^+ T cells, IEL, NK cells, platelets and epithelial cells. Applications Reported: The HM alpha2 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional assays. Applications Tested: The HM alpha2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD49b (Integrin alpha 2) Monoclonal antibody specifically detects CD49b (Integrin alpha 2) in Mouse samples. It is validated for Flow Cytometry, Functional Assay