CLIP Mouse anti-Human, Brilliant Violet 786, Clone: CerCLIP, BD Optibuild™

Description

The CerCLIP monoclonal antibody specifically recognizes the class II associated invariant chain peptides (CLIP). CLIP is the remaining segment of class II invariant chain (Ii) after proteolytic degradation which stays associated with HLA-DR and represents a final intermediate in the removal of invariant chain from class II molecules during antigen processing. The removal of CLIP and subsequent loading of the antigenic peptide is facilitated by the non-classical class II molecule HLA-DM. CLIP can be detected, in association with HLA-DR, on the surface of T2DR3, a mutant cell line lacking HLA-DM.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.