CLIP Mouse anti-Human, BUV395, Clone: CerCLIP, BD Optibuild™

Description

The CerCLIP monoclonal antibody specifically recognizes the class II associated invariant chain peptides (CLIP). CLIP is the remaining segment of class II invariant chain (Ii) after proteolytic degradation which stays associated with HLA-DR and represents a final intermediate in the removal of invariant chain from class II molecules during antigen processing. The removal of CLIP and subsequent loading of the antigenic peptide is facilitated by the non-classical class II molecule HLA-DM. CLIP can be detected, in association with HLA-DR, on the surface of T2DR3, a mutant cell line lacking HLA-DM.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.