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Applied Biosystems™ Custom TaqMan™ SNP Genotyping Assay, human
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Description
Includes
Two unlabelled PCR primers: forward and reverse (primers at 900nM final concentration); 1 VIC dye: MGB-labeled probe detects the Allele 1 sequence (probes at 200nM final concentration); 1 6FAM dye: MGB labeled probe detects the Allele 2 sequence (probes at 200nM final concentration)
Create assays by submitting confidential target sequences to Applied Biosystem's secure assay design pipeline using the Custom TaqMan Assay Design Tool. This pipeline has generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data. These assays use TaqMan minor groove-binding (MGB) probes for superior allelic discrimination, improved signal-to-noise ratios, and design flexibility. All human SNP genotyping assays are functionally tested on 20 unique DNA samples to ensure assay amplification and sample clustering. These custom assays are designed, synthesized, formulated, optimized, and quality control tested. They are ideal for genotyping applications including screening, association, candidate region, candidate gene, or fine-mapping studies.
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'-nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.
Custom TaqMan SNP Genotyping Assays can be easily designed at no extra charge by submitting target sequences confidentially to our secure Custom Assay Design Tool.
Benefits:
- Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
- Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
- Flexible—obtain assay designs for any SNP in the human genome using our secure Custom Assay Design Tool, at no extra charge
- Tested—all custom human assays are subjected to a functional test on 20 unique DNA samples
Approximate ship time
4–6 days in North America and 6–10 days in Europe
The free Custom Assay Design Tool can generate assays for the detection of SNPs, MNPs, and indels of up to six bases. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).
All assay designs are the product of our industry-leading bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)
Spécification
Spécification
| Concentration | 40X |
| Content And Storage | 1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers). Store at -15 to -25°C. |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals. |
| Green Features | Sustainable packaging |
| No. of Reactions | 1500 (384-well); 300 (96-well) |
| Product Line | TaqMan™ |
| Product Type | Custom TaqMan Genotyping |
| Quantity | S (300 reactions), made to order |
| Shipping Condition | Room Temperature |
| Sample Type | Human |
For Research Use Only. Not for use in diagnostic procedures.
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