PeproTech CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK. Preparation: Produced from sera of goats immunized with highly pure recombinant Murine SDF-1alpha (CXCL12). Anti-Murine SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Murine SDF-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SDF-1alpha (500-P164G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SDF-1alpha. Western Blot: To detect Murine SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P164GBT-1MG will be provided as 2 x 500 μg

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.