PeproTech CXCL6 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GCP-2 (CXCL6). Anti-Human GCP-2 (CXCL6)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GCP-2 (CXCL6) (500-P120) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GCP-2 (CXCL6). Western Blot: To detect hGCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P120BT-1MG will be provided as 2 x 500 μg

CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).