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Description
CyDye fluors are versatile fluorophores designed for a broad range of applications. CyDye fluors produce high sensitivity and highly reproducible performance at a biologically suitable pH range of 3 to 10. The use of multicolor CyDye flours enables multiplexing without crosstalk for high accuracy.
Cy5.5 NHS Ester provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides.
- Readily dissolves in and produces a stable signal in aqueous buffers (no need for organic solvents)
- High purity and thorough QC testing generate high levels of chromophore and reactive dye content
- Narrow excitation and emission bands result in discrete signals from each fluor
- Demonstrates larger polarization range than conventional fluors
- Fluorescence lifetime of 2.8 ns
- High fluorescence intensity
- Low non-specific binding
Specifications
Specifications
| Format | Powdered |
| For Use With (Application) | For labelling via amine groups. CyDyes are suitable for use in applications such as fluorescence resonance energy transfer (FRET), time resolved- fluorescence resonance energy transfer (TR-FRET), and fluorescence polarization (FP) |
| Quantity | 10 mg |
| Product Type | Cy5.5 NHS Ester |
| Kit Contents | Foil packs of premeasured dye |
| pH Range | 3 to 10 |
| Molecular Weight (g/mol) | 1128.42 |
| Solubility | Soluble in cold water and hot water |
| Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
| Label or Dye | Cy5.5 |
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Safety and Handling
- Danger
- Acute toxicity Category 4
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H317-May cause an allergic skin reaction.
- H332-Harmful if inhaled.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P310-Immediately call a POISON CENTER/doctor/.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Cy5.5 (NHS ester)
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