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Cytochrome c Antibody (CYCS/1010), Novus Biologicals™

Mouse Monoclonal Antibody

265.00€ - 474.00€

Especificaciones

Antigen Cytochrome c
Clone CYCS/1010
Concentration 0.2mg/mL
Dilution Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
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Productos 2
Código de producto Marca Quantity Precio Cantidad y disponibilidad  
Código de producto Marca Quantity Precio Cantidad y disponibilidad  
18108308
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Novus Biologicals
NBP2-44446-0.02MG
0.02 mg
265.00€
0.02mg
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18171878
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Novus Biologicals
NBP2-44446-0.1MG
0.1 mg
474.00€
0.10mg
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Descripción

Descripción

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.
Especificaciones

Especificaciones

Cytochrome c
0.2mg/mL
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Unconjugated
Mouse
Apoptosis, Cellular Markers, Cholesterol Metabolism, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
CYCHCS, cytochrome c, cytochrome c, somatic, THC4
CYCS
IgG2b κ
Protein A or G purified
Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
CYCS/1010
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Monoclonal
Purified
RUO
Human, Rat
P99999
54205
Recombinant full-length human CYCS protein
Primary
Store at 4C.
15 kDa
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