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DAB EASY tablets, for immunohistochemistry, 10 mg/tablet

Product Code. 10778932 Shop All Thermo Scientific Chemicals Products
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Regulatory: This product is an isolated intermediate transported in accordance with Regulation (EC) 1907/2006 (REACH). All customers must complete a declaration before delivery.
Product Code. 10778932 Supplier Thermo Scientific Acros Supplier No. 328005000
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This item is not returnable. View return policy
Regulatory: This product is an isolated intermediate transported in accordance with Regulation (EC) 1907/2006 (REACH). All customers must complete a declaration before delivery.

Description

DAB, or 3,3-diaminobenzidine tetrahydrochloride, EASY tablets for immunohistochemistry, CAS # 7411-49-6, is a chromogenic substrate used to observe peroxidase activity. In immunohistochemistry, DAB is used to catalyze peroxidase-bound antibodies in order to visualize protein presence. | CAS: 7411-49-6

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • 3,3′-Diaminobenzidine is a derivative of benzene and the precursor to polybenzimidazole

Applications

  • DAB is mainly used to visualize peroxidase activity. During this chemical reaction, DAB is a hydrogen donor in the presence of peroxide. When DAB is oxidized, an insoluble brown end-product is produced.
  • DAB has been used for staining and oxidative detection of mitochondria
  • It has also been utilized for electron microscopy applications
TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material DAB EASY tablets
Physical Form Tablets
CAS 7411-49-6, 10043-35-3
Packaging Plastic bottle
Solubility Solubility in water: soluble.
Quantity 500 mg
MDL Number MFCD00007725

Frequently Asked Questions (FAQs)

How should I reconstitute DAB EASY tablets, for immunohistochemistry, 10 mg/tablet (Cat. No. 328005000)?

1. Remove the bottle from the refrigerator and allow to stand at room temperature (~20 degrees C) for 15-30 min.
2. Dissolve five tablets containing 10 mg DAB in 10 mL of distilled water at room temperature. Do not use metal forceps during handling of the tablets, since metal ions catalyze the oxidation process. Protect the solution from direct sunlight.
3. Some fine particulate matter will remain in the suspension even after the solution is thoroughly mixed. This will not affect your results, however if preferred, the solution can be filtered using Whatman No. 2 paper.
4. Add 4 urea peroxide tablets (Acros Cat. No. 32807) to 10 mL of the substrate solution. Alternatively add 40 µL 30% hydrogen peroxide (H2O2) and mix. This solution should be used within 2 hours.

Safety and Handling

compliance-icons
Product Identifier
  • DAB EASY tablets
 Signal Word
  • Danger
 Hazard Category
  • Germ cell mutagenicity Category 2
  • Carcinogenicity Category 1B
  • Reproductive toxicity Category 1B
 Hazard Statement
  • H360FD-May damage fertility. May damage the unborn child.
  • H341-Suspected of causing genetic defects.
  • H350-May cause cancer.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
 Supplemental information
  • MIXTURE LIST-Contain: Boric acid (H3BO3),3,3'-Diaminobenzidine tetrahydrochloride
SDS

RUO – Research Use Only

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