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Macherey-Nagel™ Detoxification Buffer ERB
Description
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
- Ultra-fast procedure simplifies the work-flow
- High-throughput — 96-well plates allow for easy automation and supports plasmid screening
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications
Specifications
| Chemical Name or Material | Detoxification buffer ERB |
| CAS | 102-71-6 |
| Color | Colorless |
| For Use With (Equipment) | NucleoSpin Plasmid/96 Plasmid Transfection-grade full and core kit |
| pH | 5.5 to 6.5 |
| Physical Form | Liquid |
| Quantity | 1000 mL |
| Formulation | Triethanolamine; 2-Propanol; Water |
| Odor | Alcoholic |
| Solubility Information | Soluble in Water |
Safety and Handling
- Buffer ERB (1000 mL)
- Warning
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Specific target organ toxicity Category 3
- H226-Flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- MIXTURE LIST-Contains : triethanolamine, 2-propanol
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