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Thermo Scientific Chemicals DL-1,4-Dithiothreitol, 99%, for biochemistry

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Beschreibung

1,4-Dithiothreitol, CAS # 3483-12-3, is a reducing agent commonly used to reduce disulphide bonds in protein studies, such as western blot SDS-page electrophoresis. | CAS: 3483-12-03 | C4H10O2S2 | 154.25 g/mol

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General description

  • 1,4-Dithiothreitol (DTT or Cleland's reagent) reduces disulfide bonds in proteins, leading to loss of native conformation
  • DTT is not stable in solution and should be prepared in small aliquots prior to use; the use of EDTA in the solution can increase the half-life of DTT
  • Typically used at a final concentration of 0.1M for protein stabilization; recommended to create a 1M stock

Applications

  • Protects thiol groups from oxidation and acts as a chemical chelator in solution; maintains proteins in the denatured state
  • Common applications include SDS-page electrophoresis and protein isolation
TRUSTED_SUSTAINABILITY

Chemische Identifikatoren

CAS 12-3-3483
MDL Number MFCD00004877
InChI Key VHJLVAABSRFDPM-IMJSIDKUSA-N
Synonym dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
PubChem CID 446094
ChEBI CHEBI:42170
IUPAC Name (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
SMILES C(C(C(CS)O)O)S

Spezifikation

Absorbance (1M soln. in water), (1 M soln. in water) at 0nm, 0.035 max. at 500nm
Melting Point 39°C to 44°C
Color White
Infrared Spectrum Authentic
Assay Percent Range 0.99
Molecular Formula C4H10O2S2
Linear Formula HSCH2CH(OH)CH(OH)CH2SH
Quantity 5 g
Beilstein 01, III, 2360
Merck Index 15, 3419
Transmittance 99% min. (c=5, 660nm, water) 1cm cell
Molecular Weight (g/mol) 154.24
Formula Weight 154.24
Percent Purity ≥98.5%
Grade Biochemical
Physical Form Powder
Chemical Name or Material DL-1, 4-Dithiothreitol
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Sicherheit und Handhabung

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Produktidentifikator
  • DL-1,4-Dithiothreitol
 Signalwort
  • Warning
 Gefahrenkategorie
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Gefahrenhinweis
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H302-Harmful if swallowed.
 Sicherheitshinweis
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
Sicherheitsdatenblatt (SDS)

RUO – Research Use Only

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