missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
GREENER_CHOICE

Código de producto. 15683748 Tienda IBA Lifesciences Productos
Click to view available options
Vector:
pASG-IBA103
pASG-IBA105
pASG-IBA123
pASG-IBA143
pASG-IBA145
pASG-IBA162
pASG-IBA164
pASG-IBA167
pASG-IBA168
pASG-IBA23
pASG-IBA3
pASG-IBA43
pASG-IBA45
pASG-IBA5
pASG-IBA62
pASG-IBA64
pASG-IBAwt1
Tamaño de la unidad:
5µg
Este artículo no se puede devolver. Vea la política de devoluciones

Código de producto. 15683748

Marca: IBA Lifesciences 54145001

Please to purchase this item. Need a web account? Register with us today!

Este artículo no se puede devolver. Vea la política de devoluciones

Descripción

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.

  • The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
  • The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
  • The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
  • Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Especificaciones

Inducing Agent Inducible with anhydrotetracycline
Marker Gene Ampicillin
Promoter Tet
Product Type Vector
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Species E.coli
Protein Tag His Tag (6x), Twin-Strep-tag
Cloning Method Direct Cloning
Concentration 250 ng/μL
Primer Forward
Quantity 5 μg
Vector pASG-IBA145
For Use With (Application) Vector for recombinant expression in E. coli
Mostrar más Mostrar menos
Corrección del contenido de un producto

Proporcione sus comentarios sobre el contenido del producto rellenando el siguiente formulario.

Título del producto

Al hacer clic en Enviar, acepta que Fisher Scientific se ponga en contacto con usted en relación con los comentarios que ha proporcionado en este formulario. No compartiremos su información para ningún otro fin. Toda la información de contacto proporcionada se mantendrá de acuerdo con nuestra Política de Privacidad. Política de privacidad.

Gracias por ayudarnos a mejorar nuestra web. Su comentario ha sido enviado