Invitrogen™ Estrone Competitive ELISA Kit

ELISA

The Multispecies Estrone ELISA quantitates estrone in fecal extracts,urine,and tissue culture media.

Principle of the method The Estrone Competitive ELISA research-use-only kit is designed to quantitatively measure estrone present in extracted dried fecal samples,urine,and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone,estrone-3-sulfate,and estrone 3-glucuronide in urine and fecal samples with almost equal affinity,allowing for non-invasive testing of this steroid. An estrone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.