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Gibco™ ExpiSf™ Expression System Starter Kits

Product Code. 15859126
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15859126 Kit with Erlenmeyer Flasks Each
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Product Code. 15859126 Supplier Gibco™ Supplier No. A38841

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Description

The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.

The ExpiSf Expression System offers

  • A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
  • Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
  • Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
  • A fast, simplified workflow that allows you to obtain protein in as little as 6–10 days; half the time of traditional protocols
  • Scalable expression that can be applied to cultures from 24-deep well plates to >3 L shake flasks
TRUSTED_SUSTAINABILITY

Specifications

Type Insect Cell Protein Expression System
Cloning Method Restriction Enzyme/MCS
Protein Tag Untagged
Delivery Type Baculovirus
Quantity 1 kit
Content And Storage

• 1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C
• 4 x 1 mL ExpiSf Enhancer, 2°C to 8°C
• 1 mL ExpiFectamine Sf Transfection Reagent, 2°C to 8°C
• 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen
• 500 mL Opti-MEM, 2°C to 8°C
• 10 μg pFastBac1 vector, 2°C to 8°C
• 4 ng pFastBac1-Gus control vector, 2°C to 8°C
• 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, -80°C
• 24 x 125 mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature
Format Kit with Erlenmeyer Flasks
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Cell Line Sf9, SFM Adapted
Product Line ExpiSf
Promoter Polyhedrin
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For Research Use Only. Not for use in diagnostic procedures.

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