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Description
- A convenient method to label proteins with varying amounts maleimide for crosslinking or conjugation with a thiol/sulfhydryl containing moiety
- Sulfo-SMCC contained in this kit readily reacts with the primary amines on a protein to form a covalently attached maleimide group which can be further reacted with a reactive thiol or free cystine to create diverse bioconjugates
- Thermo Scientific™ Sulfo-SMCC is a heterobifunctional crosslinker which contains a sulfonated N-hydroxysuccinimide (NHS) ester and maleimide group that allow covalent conjugation of amine- and thiol/sulfhydryl-containing molecules
- Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins
- This kit contains sufficient reagents for five labeling reactions of up to 100 μL sample with a concentration of 1-10 mg/mL
- Sulfo-SMCC reagent readily dissolves in water
Specifications
Specifications
| Quantity | 1 Kit |
| Content And Storage | Contains 5 vials of sulfo-SMCC, 5 Zeba Dye & Biotin Removal columns, and BupH™ Phosphate Buffered Saline pack. Store sulfo-SMCC desiccated at –20°C, Zeba Dye and Biotin Removal columns at 2–8°C. |
| Chemical Reactivity | Amine-to-sulfhydryl |
| Labeling Method | Chemical Labeling |
| Shipping Condition | Wet Ice |
| Reactive Moiety | Sulfo-NHS, Maleimide |
| Product Line | EZ-Link |
Product Title
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