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Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-Biotinylation Kit

Product Code. 10687714 Shop All Thermo Scientific Products
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8 reactions
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1 set
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Product Code. 10687714 Supplier Thermo Scientific™ Supplier No. 21925

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Description

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.
TRUSTED_SUSTAINABILITY

Specifications

Description EZ-Link Micro Sulfo-NHS-Biotinylation Kit
Product Type Micro Sulfo-NHS-Biotinylation Kit
Quantity 8 reactions
Content And Storage Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody
• EZ-Link Sulfo-NHS-Biotin, 8 x 1 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 2 mL, 10 columns

Store biotin reagent at -20°C. Store remaining kit components at 4°C.
Labeling Scale 100 μg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Spacer Pegylated
Labeling Target Antibodies, Proteins

Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

For Research Use Only. Not for use in diagnostic procedures.

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