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Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
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Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction setup and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq Buffer, which includes 20 mM MgCl2
DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.
Features
- Robust amplification with minimal optimization
- High yields of PCR products
- Higher sensitivity compared to conventional Taq DNA polymerase
- Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
- Incorporates modified nucleotides, but does not incorporate dUTP
- Direct loading of PCR product on gel with green format
Applications
- Routine PCR amplification of DNA fragments up to 6 kb
- High throughput PCR
- Genotyping
Notes
- For applications requiring PCR product analysis by absorbance or fluorescence excitation, colorless 10X DreamTaq Buffer (Cat. No. B65) is recommended for purifying PCR products before analysis.
Specifications
Specifications
| Concentration | 5 U/μL |
| Content And Storage | • DreamTaq DNA Polymerase (5 U/μL), 5 x 100 μL • 10X DreamTaq Buffer with 20 mM MgCl2, 10 x 1.25 mL Store at -20°C. |
| GC-Rich PCR Performance | Low |
| Polymerase | DreamTaq DNA Polymerase |
| Reaction Speed | Standard |
| Quantity | 5 x 500 Units |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR, Genotyping |
| Fidelity (vs. Taq) | 1X |
| Hot Start | No |
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For Research Use Only. Not for use in diagnostic procedures.
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