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Description
GeneRuler 50bp DNA Ladder is recommended for sizing and for approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler 50bp DNA Ladder can be labeled radioactively with T4Polynucleotide Kinase.
The ladder is supplied with 6X DNA Loading Dye.
- Supplied with loading dye for sample DNA
- Ideal for both DNA sizing and approximate quantification
- Sharp bands
- Bright reference bands (given in red)
Specifications
Specifications
| Concentration | 6X |
| Gel Type | Agarose |
| Label or Dye | Xylene Cyanol FF, Bromophenol Blue, Orange G |
| Ready to Load | No |
| Size Range | 50 to 1000 bp |
| Gel Compatibility | Agarose Gels |
| Quantity | 50 μg |
| Product Line | GeneRuler |
| Product Type | DNA Ladder |
Frequently Asked Questions (FAQs)
The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.
For Research Use Only. Not for use in diagnostic procedures.
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