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Thermo Scientific™ SP6 RNA Polymerase (20 U/μL)

Product Code. 10344450 Shop All Thermo Scientific Products
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2,000 units
Unit Size:
2000 units
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Product Code. 10344450 Supplier Thermo Scientific™ Supplier No. EP0131

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71.40 EUR valid until 2026-03-27
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Description

Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Thermo Scientific™ Bacteriophage SP6 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

TRUSTED_SUSTAINABILITY

Especificaciones

Polymerase SP6 RNA Polymerase
Quantity 2,000 units
Concentration 1X

For Research Use Only. Not for use in diagnostic procedures.

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