Learn More
Gibco™ Fetal Bovine Serum, Tet system approved, USDA-approved regions
Description
Tet system-approved FBS is formulated for cell culture applications using tetracycline- and tetracycline derivative-regulated gene expression systems. Cell-based assays that detect the presence of tetracycline and tetracycline analogs are used to select FBS that delivers the highest range of induction.
- Designed to ensure the absence of detectable tetracycline and its derivatives that may interfere with accurate regulation of gene expression
- Functional testing provides confidence that unintended tetracyline gene expression will not result
- Sera category: specialty
Specifications
Specifications
| Certifications/Compliance | ISO 13485 Certified |
| Classification | Value |
| Content And Storage | Store at <-10°C. |
| Species | Cattle/Bovine |
| Cell Type | Use for a broad range of cell types, especially sensitive cell lines |
| Age | Fetal |
| Country of Origin | Mexico |
| Format | One Shot |
| Specialty Type | Tet-System Approved |
| Product Type | Fetal Bovine Serum |
| Show More |
Frequently Asked Questions (FAQs)
- Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
- Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
- There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.
Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.
1. Pellerin, et al., Bioengineering, published in 2021.
While competitors may use HPLC or other assays to determine the presence of tetracycline, we developed a cell-based assay because we wanted to verify that the tetracycline present, if any, in this product would not be enough to alter the experiment. The only way to confirm this is to actually demonstrate the efficacy of the serum in a dish with cells (cell-based assay).
Fetal Bovine Serum, Tet system approved is not tetracycline-free, and no serum can be absolutely confirmed to be tetracycline-free without altering the performance of the product. We developed a cell-based assay to confirm that tetracycline, if present, is not in high enough concentrations to alter Tet experiments.
Sorry, we do not offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated.
Since our sera are not pre-aged before filtration, turbidity or flocculent debris may develop upon thawing or storage. This flocculence is a normal characteristic of the serum and does not adversely affect performance of the serum.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.