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  6. Gibco™ Antibiotic-Antimycotic (100X) 

Gibco™ Antibiotic-Antimycotic (100X)
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Product Code. 11570486
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Product Code. 11570486 Supplier Gibco™ Supplier No. 15240062

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Description

Solution contains penicillin, streptomycin, and Gibco Amphotericin B to prevent cell culture contamination.

Gibco™ Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 μg/mL of streptomycin, and 25 μg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast.

Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture
Concentration 100 X
Culture Type Mammalian Cell Culture
Form Liquid
Product Type Antibiotic-Antimycotic
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
Quantity 100 mL
Shipping Condition Dry Ice

Frequently Asked Questions (FAQs)

Can I use pen/strep or other antibiotics for episomal reprogramming of cells?

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.
What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Should I directly add 5 mL of Antibiotic-Antimycotic (100X) to 500 mL cell culture medium or should I dilute the Antibiotic-Antimycotic (100X) 100 times and then add 5 ml of the diluted Antibiotic-Antimycotic to 500 ml of cell culture medium?

The accurate way would be to dilute 5 mL of Antibiotic-Antimycotic (100X) into 495 mL of medium. Please note that the volume of medium in a 500 mL medium bottle is not exactly 500 mL. It could range from 500.5 - 510.5 mL.

Can antibiotics be used with StemFlex Medium to prevent contamination during gene editing/flow sorting experiments?

Yes. We use Antibiotic-Antimycotic (Cat. No. 15240062) for additional protection from contamination.
How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Safety and Handling

compliance-icons
Product Identifier
  • Antibiotic Antimycotic
 Signal Word
  • Danger
 Hazard Category
  • Reproductive toxicity Category 1
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 2
 Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H360-May damage fertility or the unborn child.
  • H371-May cause damage to organs.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: penicillin G sodiumsalt, streptomycin sulphate

For Research Use Only. Not for use in diagnostic procedures.

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