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Gibco™ CD Hybridoma AGT™ Medium

Product Code. 10710204
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Product Code. 10710204 Supplier Gibco™ Supplier No. 12372025

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This item is not returnable. View return policy

Animal origin-free, chemically defined, protein-free medium

Gibco CD Hybridoma Medium is specifically developed for the ability to support the growth of hybridomas for antibody production.

  • Ability to support suspension and stationary hybridomas and myelomas
  • Formulated without L-glutamine for stability
  • Animal origin-free, protein-free and chemically defined formulation
  • Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas

  • Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco CD Hybridoma Medium provides high cell densities and IgG production
  • Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate
Formulated without L-glutamine for stability
  • Gibco CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use
  • GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
Animal origin-free, protein-free and chemically defined formulation
  • Gibco CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest
  • Gibco chemically defined media contain no proteins, hydrolysates or components of unknown composition
Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
  • The AGT format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation
  • Complete reconstitution instructions are available in the product manual

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Wet ice

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Myeloma Cells, Hybridomas
Classification Animal Origin-free, Chemically-defined, Protein-free
Form Powder (AGT)
Product Type Hybridoma Medium
Serum Level Serum-free
Sterility Non-sterile
With Additives Sodium Pyruvate, Sodium Bicarbonate
Without Additives No Glutamine, No Phenol Red
Product Line AGT, Gibco
Quantity 1 L
Shipping Condition Wet Ice
Endotoxin Level ≤10 EU/mL, ≤ 10 EU/ml
Content And Storage Storage conditions: 2-8°C, dark and dry
Shipping conditions: Gel packs
Shelf life: 24 months from date of manufacture
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What are the main benefits of using AGT?

Complete formulations
- Homogeneous distribution of minute components
- Complete complex formulations in a dry format, including serum-free, protein-free, and chemically defined nutrient media
- Pre-adjusted to the appropriate pH and osmolality
- Require only standard supplementation with L-glutamine for L-glutamine dependent systems
- Can be customized for modified catalog and customers' proprietary formulations

Granular format
- Dissolves instantly for faster media preparation times
- Dust-free granules reduce mess and environmental contamination
- Performance equivalent to liquid media
- Workflow improvement
- Helps reduce total cycle costs due to lower number of components, decreasing time involved in raw material planning, procurement, and testing
- Faster and easier media preparation, with less potential for error
- Easy to scale up from research through production, in batch sizes from 2 kg to 6,000 kg
- Available in various packaging options

What is AGT?

Granulated cell culture media is an easy-to-use, granular form of dry media that is produced by applying fluid bed granulation technology to cell culture nutrient media manufacturing. The patented process allows production of complete formulations of a variety of serum-free, protein-free, and chemically defined media in a dry format.

AGT offers the benefits of liquid media without the cost, storage, and transportation issues. It is a complete medium that is pH and osmolality pre-adjusted. The granules dissolve instantly for faster media preparation time than conventional dry powder media. AGT media can therefore help reduce total cycle costs, decreasing time involved in raw material planning, procurement, and testing as well as media preparation. These benefits are all realized while maintaining comparable cell growth to liquid media.

For Research Use Only. Not for use in diagnostic procedures.

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