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Gibco™ DMEM, high glucose, no glutamine

Code produit 11500416 Tous les produits Gibco Produits
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1 x 500 mL
Conditionnement:
500mL
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Code produit Quantity Conditionnement
11500416 1 x 500 mL 500mL
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Code produit 11500416 Fournisseur Gibco™ Code fournisseur 11960044
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Description

Requires

Supplementation

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, Phenol Red

Without: L-glutamine, Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Informations sur la commande

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

TRUSTED_SUSTAINABILITY

Spécification

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 1X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose, Phenol Red
Without Additives No Glutamine, No HEPES, No Sodium Pyruvate
For Use With (Application) Mammalian Cell Culture
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 1 x 500 mL
Shipping Condition Ambient
Format Bottle
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Environment CO2
Culture Type Mammalian Cell Culture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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