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Gibco™ Ham's F-10 Nutrient Mix
Description
Requires
Supplementation
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
This Ham's F-10 is modified as follows:
With: Phenol Red, L-glutamine
Without: HEPES
The complete formulation is available.
Using Ham's F-10
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Cell Line | CHO, COS-7, and rat prostate epithelial cells |
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | Ham's F-10 Nutrient Mixture |
| Sterility | Sterile-filtered |
| With Additives | High Glucose, Glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | No HEPES |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
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For Research Use Only. Not for use in diagnostic procedures.
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