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  7. Gibco™ Medium 199, Earle's Salts 

Gibco™ Medium 199, Earle's Salts

Product Code. 11554426 Shop All Gibco Products
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Quantity:
500 mL
10 x 500 mL
With Additives:
Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Low Glucose, Glutamine, Phenol Red
Unit Size:
5000mL
500mL
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Product Code. Quantity With Additives Unit Size
11554426 10 x 500 mL Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate 5000mL
15440554 500 mL Low Glucose, Glutamine, Phenol Red 500mL
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Product Code. 11554426 Supplier Gibco™ Supplier No. 31150030
 Restricted Item
Estimated Shipment: 13-11-2026
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Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

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Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line Rat epithelial
Cell Type Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Concentration 1X
Form Liquid
Product Type Medium 199
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 10 x 500 mL
Osmolality 290 - 310 mOsm/kg
Content And Storage Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Culture Environment CO2
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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