Invitrogen™ HDAC1 Polyclonal Antibody

Description

PA1-860 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells. PA1-860 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluoresence, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~62 kDa protein representing HDAC1 from HeLa cell lysate. Immunocytochemical staining of HDAC1 in HeLa cells with PA1-860 results in nuclear staining. The PA1-860 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.