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Proteintech™ Human Fetuin-A ELISA Kit

Product Code. 16871506 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
Product Code. Target Species unitSize
16871506 Human 96 tests
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Product Code. 16871506

Brand: Proteintech™ KE0009796T

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This item is not returnable. View return policy

Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous Fetuin A

  • Recognizes human Fetuin A
  • Fetuin A (alpha2-HS glycoprotein, AHSG) is a member of cystatin superfamily of protease inhibitors
    • Highly expressed glycoprotein in various fetal tissues, mainly expressed by liver in adults
    • Fetuin A is an extracellular inhibitor of transforming growth factor β is a profibrogenic stimulus in liver disease
    • May be beneficial serum biomarker in detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease
    • Involved in functions like endocytosis, brain development and formation of bone tissue
    • Fetuin A exerts effects on cardiovascular system by inhibiting insulin signaling and inducing insulin resistance
      • Also contributes to onset of atherosclerosis and inhibits calcium deposition and protects from vascular calcification

Process:

  • Antibody specific for Fetuin A pre-coated onto microwells
  • Fetuin A protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for Fetuin A is added to detect the captured Fetuin A protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number NP_001613
Assay Range 0.313 to 20 ng/mL
Assay Sensitivity 0.1 ng/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 197
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P02765
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Safety and Handling

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Product Identifier
  • 96TEST Human Fetuin-A ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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