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Proteintech™ Human GRP ELISA Kit

Product Code. 16841486 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. Target Species unitSize
16841486 Human 96 tests
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Product Code. 16841486 Supplier Proteintech™ Supplier No. KE0007096T

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Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous GRP

  • Recognizes human GRP
  • GRP (Gastrin-releasing peptide) is normally formed by mucosal cells in gastric antrum and by D cells of pancreatic islets, main function is to stimulate secretion of HCl by gastric mucosa
    • HCl inhibits gastrin formation
    • 148 amino acid preproprotein, following cleavage of signal peptide is further processed to produce either 27 amino acid gastrin-releasing peptide or 10 amino acid neuromedin C
    • Smaller peptides regulate numerous functions of gastrointestinal and CNS (gastrointenstinal hormones, smooth muscle cell contraction, epithelial cell proliferation)
      • Peptides likely to play role in human cancers (lung, colon, stomach, pancreas, breast, prostate)

Process:

  • Antibody specific for GRP pre-coated onto microwells
  • GRP protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for GRP is added to detect the captured GRP protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number NP_001012530
Assay Range 0.313 to 20 ng/mL
Assay Sensitivity 0.03 ng/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 2922
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P07492
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Safety and Handling

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Product Identifier
  • 96TEST Human MMP-2 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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