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Description
Its mode of action is the inhibition of protein synthesis, by inducing the misreading of the m-RNA template in the prokaryote, (E. coli, at 100 mg/mL), lower eukaryotes (e.g., yeast, at 200mg/mL) and higher eukaryotes (e.g., mammalian cells in culture). It selectively penetrates cells that have been rendered permeable by virus infection and combined with its potency to inhibit translation, it is an effective antiviral agent. Hygromycin B can be used as a dual-selection antibiotic when used in conjunction with G418 or Zeocin™ for cells transfected with two different vectors.
- Blocks polypeptide synthesis and inhibits elongation. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template.
- When using this product for mammalian cell selection the working concentration is generally between 50mg/mL and 1mg/mL, but at times may go as high as 1 mg/mL.
- The appropriate concentration for use must be determined empirically for each cell type.
Specifications
Specifications
| Physical Form | Powder |
| CAS | 31282-04-9 |
| Melting Point | 160°C to 180°C (literature) |
| Quantity | 1 g |
| Molecular Formula | C20H37N3O13 |
| Synonym | O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine |
| Solubility Information | Freely soluble in water (50 mg/mL), methanol,buffer solution and ethanol. Practically insoluble in less polar solvents. |
| InChI Key | GRRNUXAQVGOGFE-BBMONYMYSA-N |
| SMILES | CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N |
| IUPAC Name | (3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol |
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Safety and Handling
- Hygromycin B
- Danger
- Acute toxicity Category 2
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 1
- Skin sensitiser Category 1
- H300 + H330-Fatal if swallowed or if inhaled.
- H318-Causes serious eye damage.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H317-May cause an allergic skin reaction.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P320-Specific treatment is urgent (see on this label).
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
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