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Invitrogen™ I-Block™ Protein-Based Blocking Reagent
Description
- Biotin-free
- Tested in assays using Tropix substrates and alkaline phosphatase conjugates
- Useful as blocking reagent in membrane-based and immunoassay applications
- Suggested working
- concentration is 0.2% (w/v) for detection of nucleic acids on neutral or positively-charged nylon membranes and for immunoassays and protein detection on membranes (nitrocellulose, PVDF, or neutral nylon)
- Concentration of 3% is recommended for protein detection on positively-charged nylon membrane (such as TropilonPlus™ membrane)
Preparation:
Prepared in either Tris- or phosphate-buffered saline buffer with heating (40° to 50°C).
Applications:
Can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems.
Specifications
Specifications
| Chemical Name or Material | Reagent |
| For Use With (Equipment) | Western-Light™ Detection System, Western-Star™ Detection System, Southern-Star™ Detection System, ELISA-Light™ Detection System, Southern-Light™ Detection System, Western-Light Plus™ Detection System |
| Product Line | I-Block, NovaBright |
| Quantity | 30 g |
For Research Use Only. Not for use in diagnostic procedures.
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