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Invitrogen™ Pichia Expression Kit, original kit

Product Code. 10768244
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Quantity:
1 Kit
Unit Size:
1 set
Product Code. Quantity unitSize
10768244 1 Kit 1 set
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Product Code. 10768244

Brand: Invitrogen™ K171001

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Description

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.

TRUSTED_SUSTAINABILITY

Specifications

Antibiotic Resistance Bacterial Ampicillin (AmpR)
Bacterial or Yeast Strain GS115, KM71
Content And Storage The Original Pichia Expression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5' AOX1, 3' AOX1, and α-factor sequencing primers.

Store vectors and primers at -20°C. Store strains at room temperature.
All components are guaranteed stable for 6 months when properly stored., Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months
Cloning Method Restriction Enzyme⁄ MCS
Promoter AOX1
Cell Type Yeast Cells
Format Kit
Species P. pastoris
Vector pPIC
Selection Agent (Eukaryotic) Ampillicin
Expression Mechanism Cell-Based Expression
Expression System Yeast
For Use With (Application) Protein Expression
Product Type Expression Kit
Protein Tag Untagged
Quantity 1 Kit
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For Research Use Only. Not for use in diagnostic procedures.

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