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Invitrogen™ Silencer™ Firefly Luciferase (GL2 + GL3) siRNA

Product Code. 10477874
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Product Code. 10477874 Supplier Invitrogen™ Supplier No. AM4629

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252.00 EUR valid until 2026-05-08
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Description

Silencer™ Firefly Luciferase (GL2 + GL3) siRNA

Invitrogen™ Silencer™ Firefly Luciferase (GL2 + GL3) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA.
  • Validated siRNA controls for optimizing siRNA experiments
  • Gene-specific control siRNAs provided with scrambled, negative controls
  • Functionally tested in several common cell lines
  • HPLC purified, duplexed, and ready to use Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Invitrogen™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Content And Storage Store at or below -20°C. Do not store in a frost-free freezer.
    Control Type Positive Control
    RNAi Type siRNA
    Species Firefly Luciferase
    For Use With (Application) Transfection, RNAi
    Label or Dye Unconjugated
    Product Line Silencer, Ambion
    Product Type siRNA
    Purity HPLC
    Quantity 5 nmol
    Shipping Condition Dry Ice
    Target Luciferase
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    Frequently Asked Questions (FAQs)

    How critical is it to include positive and negative controls in siRNA experiments?

    A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.
    What are the positive and negative controls I should use with my Silencer or Silencer Select siRNA experiment?

    We offer several negative and positive controls for both Silencer and Silencer Select siRNA.

    For Silencer siRNA positive control:
    - Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
    - Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
    - Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
    - Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
    - Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)

    If working with Fluorescently labeled Silencer siRNA:
    - Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
    - Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)

    If working with Silencer siRNA in vivo ready:
    - Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

    For Silencer siRNA negative controls:
    - Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
    - Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
    - Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
    - Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
    - Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)

    If working with Fluorescently labeled Silencer siRNA:
    - Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
    - Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)

    If working with Silencer siRNA in vivo ready:
    - Silencer in vivo ready (Cat. No. 4404021)

    For Silencer Select siRNA Positive control:
    - Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
    - Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)

    For Silencer Select siRNA negative control:
    - Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
    - Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)

    If working with Silencer Select siRNA in vivo ready:
    - Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)

    For Research Use Only. Not for use in diagnostic procedures.

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