Invitrogen™ Silencer™ Human DNA Damage Response siRNA Library

DNA surveillance proteins monitor DNA integrity, and they can activate cell cycle checkpoints and DNA repair pathways in response to DNA damage. This can prevent potentially deleterious mutations. The Silencer™ Human DNA Damage Response siRNA Library contains 1725 Ambion™ Silencer siRNAs targeting 575 genes for performing RNAi screens against known DNA damage response pathways. The library was designed using the most up-to-date genome databases, including the NCBI RefSeq database and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). Utilizing Ambion Silencer technology built on stringent bioinformatics to enable target specificity and siRNA potency, the Silencer Human DNA Damage Response siRNA Library is an ideal solution for focused and cost-effective RNAi screens targeting cellular pathways that sense, signal and repair DNA lesions following DNA damage.

• Comprehensive—Expedite your screens of DNA damage genes with pre-defined Silencer human DNA damage response siRNA Library
• Reliable—Increase target screening accuracy with three individual siRNAs per gene targetEffective—Reduce off target impacts at low concentrations with proven Ambion Silencer siRNA design
• Convenient—Reduce data de-convolution and accelerate target validation with our arrayed format
• Customizable—Choose the best option for your workflow demands, including additional siRNA scales and pooled formats

Why use the Silencer Human DNA Damage Response siRNA Library?

Together with careful bioinformatics selection of gene targets, Silencer siRNAs are optimized for design and high quality synthesis with guaranteed silencing. The Silencer Human DNA Damage Response siRNA Library contains our pre-designed and when available, validated siRNAs generated with maximum potency and specificity in mind so you can focus on data quality. All Silencer libraries are selected against the latest available databases and resources for up-to-date content. Full siRNA sequence information, as well as the exon targeted and each targeted gene's symbol, full name, aliases, RefSeq number, and Locus ID, plus results from any validation experiments performed using the included siRNAs is provided. Please contact us for the complete list of targets.

Silencer siRNA libraries allow you to screen efficiently and maximize data output

Ambion Silencer siRNAs are designed with potency in mind to help ensure gene knockdown at minimum concentrations. This not only helps ensure less potential for off target effects and false positive data but also minimizes false negative outcomes of siRNA screening. The Silencer Human DNA Damage Response siRNA Library contains 0.25 nmol of each siRNA, sufficient for 100 transfections (at 25 nM siRNA, 100 μL transfection volume).

Flexibility to meet your workflow needs

Thermo Fisher Scientific offers comprehensive list of pre-defined or custom siRNA libraries to human, mouse or rat genes with 1, 2, or 5 nmol of each siRNA or larger sizes. For a complete gene list, additional information or to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAilibraries@lifetech.