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Invitrogen™ Taq DNA Polymerase, native, w/W1

Code produit 10594423
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10594423 500 Unit(s) Each
10063172 100 Unit(s) Each
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Code produit 10594423 Fournisseur Invitrogen™ Code fournisseur 18038026
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Taq DNA Polymerase, native, w/W1

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The 1% solution of the detergent W-1 that is included may improve the thermostability of the enzyme when added at a final concentration of 0.05% (v/v).

Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:

  • Your choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • An enzyme that is licensed and qualified for PCR

    Applications
    Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

    Source
    Native enzyme is purified from Thermus aquaticus YT1. Unit definition
    One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
  • TRUSTED_SUSTAINABILITY

    Spécification

    Concentration 5 U/μL
    Content And Storage –20°C
    Description Taq DNA Polymerase, 500 Unit, 5μL Concentration, Colorless, 3'-A Overhang, Taq DNA Polymerase, 1X Low GC-Rich PCR Performance, Standard, Reaction Speed, Separate Components Reaction Format
    Quantity 500 Unit(s)
    For Use With (Application) Standard PCR
    Overhang 3'-A
    Includes Taq DNA polymerase (100μL), 10X PCR Buffer without magnesium (2.5mL), 50mM Magnesium chloride (1mL), 1% W-1 (1mL)
    Source Thermus aquaticus YT1

    For Research Use Only. Not for use in diagnostic procedures.

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