missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Western Blot and Nucleic Acid Membranes and Filter Paper
  5. Invitrogen™ iBlot™ 3 Transfer Stacks, mini, nitrocellulose

Invitrogen™ iBlot™ 3 Transfer Stacks, mini, nitrocellulose
GREENER_CHOICE

Product Code. 17831374
Change view
Click to view available options
Quantity:
10 Stacks
3 x 10 Stacks
Unit Size:
Each
Pack of 30
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
17831374 10 Stacks Each
17862878 3 x 10 Stacks Pack of 30
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 17831374 Supplier Invitrogen™ Supplier No. IB33002

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

iBlot 3 Transfer Stacks are used with iBlot 3 Western Blot Transfer Device for the transfer of proteins from an SDS-PAGE gel to a membrane support when performing western blot experiments.

iBlot 3 Transfer Stacks are used with the iBlot 3 Western Blot Transfer Device for the transfer of proteins from an SDS-PAGE gel to a membrane support when performing western blot experiments. iBlot 3 Transfer Stacks contain copper-fiber electrode sheets, optimized cathode and anode solid buffer gels, and a preconditioned nitrocellulose transfer membrane. They are compatible with common commercially available protein gels, including Bis-Tris, Tris-Glycine, Tris-Acetate, and Tricine chemistries. This mini-size stack (8 x 8 cm) can be used with one mini-size gel.

The iBlot 3 Western Blot Transfer System (iBlot 3 Transfer Stacks plus iBlot 3 Transfer Device) has been optimized to provide protein transfers in 3–8 minutes that produce higher quality and more reliable western blot result compared to many other transfer methods.

Nitrocellulose membrane

The nitrocellulose membrane (0.2-μm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. Proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 μg/cm2.

TRUSTED_SUSTAINABILITY

Specifications

Length (Metric) 8 cm
Width (Metric) 8 cm
Quantity 10 Stacks
Format Transfer Stack
Material Nitrocellulose
Pore Size 0.2 μm
Content And Storage Store at room temperature.
Dimensions (LxW) 8 x 8 cm (Mini)
Product Line iBlot
Sufficient For 10 Blots

Frequently Asked Questions (FAQs)

After transferring using the iBlot 3 Western Blot Transfer System, I noticed that the Bottom Stack transfer gel melted to a viscous blue solution. Why is this?

This happens if the membrane is trimmed to fit the gel size resulting in direct contact between the Top and Bottom transfer stacks. This can be avoided by maintaining the membrane size identical to the transfer stack. Transfer quality is not affected by smaller gel size compared to the membrane.

What is the Blotting Roller that is provided with the iBlot 3 Western Blot Transfer System?

The Blotting Roller is a plastic roller attached to a stainless steel handle. It is used to remove any air bubbles between the gel and blotting membrane during the assembly of the transfer stacks and gel.

Can I cut the membrane in the iBlot 3 Transfer Stacks to fit my separating gel?

No, we recommend maintaining the membrane size identical to the transfer stacks. This helps ensure that there is no direct contact between the Top and Bottom transfer stacks.

Are the PVDF and nitrocellulose membranes in the iBlot 3 Transfer Stacks compatible with fluorescent antibodies or membrane staining (e.g., LI-COR, Ponceau S)?

The PVDF and nitrocellulose membranes in the iBlot 3 Transfer Stacks are compatible with all commonly used detection methods such as staining, immunodetection, fluorescence, etc.

Can I replace the membrane in the transfer stack with my preferred membrane when using the iBlot 3 Western Blot System?

Yes, it is possible to replace the membrane provided in your transfer stack with any membrane that is compatible with western blotting. To do this, cut the alternative membrane to match the size of your gel, and wet the membrane. Then either place the alternative membrane on top of the integrated membrane, or carefully remove the integrated membrane from the gel matrix with forceps and replace it with the new membrane. Note that Thermo Fisher Scientific only supports the use of transfer stacks when they are used with the provided instructions.

Are the copper electrodes in the iBlot 3 Transfer Stacks recyclable?

To maximize the recovery of the copper from the mesh electrodes used in the iBlot 3 Transfer Stacks, we have established an agreement with a recycling center in the United States. In order to prepare the copper electrodes for recycling, please follow the instructions listed here.

I want to perform western transfer with mini gels (8 x 8 cm), but I do not have iBlot 3 Transfer Stacks (Mini). Can I use iBlot 3 Transfer Stacks (Regular) to transfer my mini gels?

It is best not to transfer a single mini gel on a regular-sized transfer stack. Although in most cases, the transfer will be fine, empty spaces on the transfer stack that are not in direct contact with the gel could potentially cause distortions across the whole surface of the membrane, including the portion in contact with the gel. It is best to have more than 50% of a membrane in contact with the gel, if possible. Our recommendation is that you transfer 2 mini gels using a Regular transfer stack.

In the iBlot 3 Top Stack, I see a white sheet beneath the transfer gel layer. What purpose does it serve?

This is the plastic separator that separates the Top Stack from the Bottom Stack. Right before assembling the transfer stack, this separator needs to be removed from the Top Stack and discarded.

Is it okay to freeze the iBlot 3 Transfer Stacks?

The iBlot 3 Transfer Stacks should not be frozen as they contain a gel matrix that will be damaged by freezing.

Can I use the iBlot 3 Transfer Stacks after the expiration date?

No, we do not recommend using the iBlot 3 Transfer Stacks after the expiration date. Use of transfer stacks after the expiration date can lead to inferior results.

What is the recommended storage condition for the iBlot 3 Transfer Stacks?

We recommend storing the iBlot 3 Transfer Stacks at room temperature.

What are the different kinds of iBlot 3 Transfer Stacks that you offer and are they available separately?

We offer iBlot 3 Transfer Stacks with integrated nitrocellulose or PVDF membranes for transferring proteins from gels onto nitrocellulose or PVDF membranes, respectively (western blotting). These transfer stacks are available in Midi and Mini format and can also be purchased separate from the iBlot 3 Western Blot Transfer Device.

Can I trim the Bottom Stack of my iBlot 3 Transfer Stack to fit my separating gel?

No, the iBlot 3 Transfer Stack should not be trimmed to fit your gel size.

Can I use the iBlot 3 Transfer Stacks and/or iBlot 3 Absorbent Pad more than once?

No, the iBlot 3 Transfer Stacks contain a finite number of ions to drive the transfer and are depleted after a single use. We also recommend discarding the iBlot 3 Absorbent Pad after every use.
Is there a limitation on the thickness of gels that can be used with the iBlot 3 Western Blot Transfer Device?

The iBlot 3 Western Blot Transfer Device is compatible with gels with a thickness of ≤1.5 mm.
Are the iBlot 3 Transfer Stacks compatible with the original iBlot and iBlot 2 transfer devices?

Unfortunately, the iBlot 3 Transfer Stacks are not compatible with the original iBlot and iBlot 2 transfer devices. The iBlot 3 Transfer Stacks are only compatible with the iBlot 3 Western Blot Transfer Device.

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.