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IFN gamma Monoclonal Antibody (AN-18), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

93.50€ - 271.00€

Specifications

Antigen IFN gamma
Clone AN-18
Concentration 0.5 mg/mL
Applications Immunoprecipitation, Immunostaining
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15278057
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Affymetrix eBioscience
14-7313-81
50 μg
93.50€
50µg
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Estimated Shipment: 24-04-2024
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15288057
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Affymetrix eBioscience
14-7313-85
500 μg
271.00€
500µg
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Estimated Shipment: 27-03-2024
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Description

Description

Description: The AN-18 antibody reacts with mouse interferon-gamma (IFN gamma), a 15-20 kDa cytokine secreted by Th1 cells. Applications Reported: The AN-18 antibody has been reported for use in immunoprecipitation, ELISPOT, and ELISA. (Please use Functional Grade purified AN-18, cat. 16-7313, for ELISPOT Capture.). Applications Tested: The AN-18 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFNg (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/mL - 1.6 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
Specifications

Specifications

IFN gamma
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
IFNG
Primary
4° C
IFNG
AN-18
Immunoprecipitation, Immunostaining
Unconjugated
Rat
Mouse
P01580
15978
IgG1 κ
Affinity chromatography
Antibody
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