PeproTech IL-7 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-7. Anti-Human IL-7-specific antibody was purified by affinity chromatography employing an immobilized Human IL-7 matrix. Sandwich ELISA: To detect Human IL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-7 (500-P27Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-7. Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.