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  7. Invitrogen™ AccuPrime™ Pfx DNA Polymerase 

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Product Code. 10472482
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Product Code. No. of Reactions unitSize
10472482 200 Reactions Each
10227242 1000 Reactions Each
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Product Code. 10472482 Supplier Invitrogen™ Supplier No. 12344024

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Description

Includes

AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

Benefits of AccuPrime Pfx DNA Polymerase

  • Greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 12 kb
  • Minimized PCR optimization

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • High-fidelity PCR
  • Hot-start PCR

Notes

  • AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
  • The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at -20°C.
Detection Method Primer-probe
GC-Rich PCR Performance Low
Polymerase AccuPrime Pfx DNA Polymerase
Reaction Speed Standard
Quantity 200 Reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 26X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang Blunt
Reaction Format Separate Components
Size (Final Product) 12 kb or less
Volume 100 μL
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For Research Use Only. Not for use in diagnostic procedures.

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