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Invitrogen™ BioPrime™ DNA Labeling System
Description
Includes
700μL 2.5X Random Primers Solution; 175μL 10X dNTP Mixture; 100μg Control DNA; 35μL Klenow Fragment (40U/μL); 500μL Stop Buffer; 1.25mL Distilled Water
Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™ DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and for in situ hybridization to chromosome spreads.
- Particularly well suited for situations in which the DNA is limited
- Requires less template DNA than nick translation
- Amplifies template to provide an increased amount of biotinylated probe
- Labels 50 to 500ng of template DNA in one reaction
Array CGH, DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Southern Blotting
Order Info
Shipping Condition: Approved for shipment on Wet or Dry Ice
Specifications
Specifications
| Content And Storage | • 700 μl 2.5X Random Primers Solution • 175 μl 10X dNTP Mixture • 100 μg Control DNA • 35 μl Klenow Fragment (40 U/μl) • 500 μl Stop Buffer • 1.25 ml Distilled Water Store at -20°C in a non-frost-free freezer. |
| Detection Method | Biotin-based |
| Label or Dye | Biotin |
| Final Product Type | Probes (Labeled DNA) |
| Labeling Target | DNA (General), Genomic DNA |
| Includes Label or Dye | Yes |
| Labeling Method | Direct Labeling |
| Product Line | BioPrime |
| Product Type | DNA Labeling System |
| Quantity | 30 Reactions |
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For Research Use Only. Not for use in diagnostic procedures.
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