missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. DNA Polymerases
  6. 10605013

Invitrogen™ DNA Polymerase I/DNase I

Product Code. 10605013
Change view
Click to view available options
Quantity:
250 U
Unit Size:
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10605013 250 U Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10605013 Supplier Invitrogen™ Supplier No. 18162016

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Includes

DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.

An optimized mixture of both enzymes for efficient nick translation of DNA

  • Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
  • Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
  • Performance and Quality Testing: Performance tested in nick translation reaction
  • Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
  • Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template•primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min. at 37°C

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.
Description DNA Polymerase
Polymerase DNA Polymerase I
Exonuclease Activity 3' - 5', 5' - 3'
Quantity 250 U
Shipping Condition Wet Ice
Hot Start No

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.