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Invitrogen™ Dynabeads™ Untouched™ Human T Cells Kit
Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes.
Brand: Invitrogen™ 11344D
835.20 EUR valid until 2024-06-28
Use promo code "21649" to get your promotional price.
Additional Details : Weight : 0.06000kg
Includes: 10mL Depletion Dynabeads and 2mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A)
Description
Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required
Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Starting Sample:
PBMC
Order Info
Shipping Condition: Room Temperature
Specifications
| T Cells (Whole Population) | |
| Cell Isolation Kit | |
| Negative Isolation | |
| 100 Isolations | |
| DYNAL™, Dynabeads™, Untouched™ | |
| Room Temperature | |
| Human |
| The Kit Contains: 10 mL Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A). Store at 2°C to 8°C. |
|
| Cells | |
| Processes ∼1 x 109 cells total | |
| >95% | |
| PBMC | |
| 1 x 107 PBMCs per isolation |
For Research Use Only. Not for use in diagnostic procedures.